CACNA1C ELISA 试剂盒
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- 抗原 See all CACNA1C ELISA试剂盒
- CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))
- 适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) and analogues was observed.
- 灵敏度
- 0.115 ng/mL
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Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C) ELISA Kit
CACNA1C 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CACNA1C ELISA试剂盒
- CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))
- 别名
- Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNA1C 产品)
- 别名
- CACH2 ELISA Kit, CACN2 ELISA Kit, CACNL1A1 ELISA Kit, CCHL1A1 ELISA Kit, CaV1.2 ELISA Kit, LQT8 ELISA Kit, TS ELISA Kit, Cav1.2 ELISA Kit, Cchl1a1 ELISA Kit, MBC ELISA Kit, MELC-CC ELISA Kit, RATIVS302 ELISA Kit, isl ELISA Kit, C-LTCC ELISA Kit, CHCACHA1C ELISA Kit, ICA ELISA Kit, cav1.2 ELISA Kit, ca(v)1.2 ELISA Kit, CAV1.2 ELISA Kit, BCR ELISA Kit, rbc ELISA Kit, calcium voltage-gated channel subunit alpha1 C ELISA Kit, calcium channel, voltage-dependent, L type, alpha 1C subunit ELISA Kit, voltage-dependent L-type calcium channel subunit alpha-1C ELISA Kit, calcium channel, voltage-dependent, L type, alpha 1C subunit S homeolog ELISA Kit, Red blood cell count ELISA Kit, RNA, 7SL, cytoplasmic 263, pseudogene ELISA Kit, CACNA1C ELISA Kit, Cacna1c ELISA Kit, cacna1c ELISA Kit, LOC100442085 ELISA Kit, cacna1c.S ELISA Kit, RBC ELISA Kit, RN7SL263P ELISA Kit
- 背景
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Gene Name: Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit
Gene Aliases: CACH2, CACN2, CACNL1A1, CCHL1A1, CaV1.2, TS, Voltage-gated calcium channel subunit alpha Cav1.2, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle
- 基因ID
- 12288
- UniProt
- Q01815
- 途径
- Hormone Transport, Carbohydrate Homeostasis
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