ATP5B ELISA 试剂盒
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- 抗原 See all ATP5B ELISA试剂盒
- ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 3.12 ng/mL - 200 ng/mL
- 最低检测浓度
- 3.12 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). No significant cross-reactivity or interference between ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) and analogues was observed.
- 灵敏度
- 5.1 ng/mL
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ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B) ELISA Kit
ATP5B 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B) ELISA KitATP5B 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ATP5B ELISA试剂盒
- ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))
- 别名
- ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5B 产品)
- 别名
- atpmb ELISA Kit, atpsb ELISA Kit, Atpsyn-beta ELISA Kit, hm:zehn0534 ELISA Kit, im:6793121 ELISA Kit, wu:fj38d01 ELISA Kit, zgc:111961 ELISA Kit, ATPMB ELISA Kit, ATPSB ELISA Kit, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide ELISA Kit, ATP synthase subunit beta, mitochondrial ELISA Kit, ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit ELISA Kit, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide S homeolog ELISA Kit, ATP5B ELISA Kit, atp5b ELISA Kit, Atp5b ELISA Kit, LOC100401662 ELISA Kit, LOC100635763 ELISA Kit, atp5b.S ELISA Kit
- 背景
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Gene Name: ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide
Gene Aliases: ATPMB, ATPSB
- 基因ID
- 171374
- UniProt
- P10719
- 途径
- Proton Transport, Ribonucleoside Biosynthetic Process
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