ATM ELISA 试剂盒
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- 抗原 See all ATM ELISA试剂盒
- ATM (Ataxia Telangiectasia Mutated (ATM))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Ataxia Telangiectasia Mutated (ATM). No significant cross-reactivity or interference between Ataxia Telangiectasia Mutated (ATM) and analogues was observed.
- 灵敏度
- 6.8 pg/mL
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Ataxia Telangiectasia Mutated (ATM) ELISA Kit
ATM 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Ataxia Telangiectasia Mutated (ATM) ELISA KitATM 适用: 小鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ataxia Telangiectasia Mutated (ATM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ataxia Telangiectasia Mutated (ATM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ataxia Telangiectasia Mutated (ATM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ataxia Telangiectasia Mutated (ATM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ataxia Telangiectasia Mutated (ATM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ataxia Telangiectasia Mutated (ATM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ATM ELISA试剂盒
- ATM (Ataxia Telangiectasia Mutated (ATM))
- 别名
- Ataxia Telangiectasia Mutated (ATM 产品)
- 别名
- ATM ELISA Kit, Atm ELISA Kit, CG6535 ELISA Kit, Dmel\\CG6535 ELISA Kit, Tefu ELISA Kit, atm ELISA Kit, atm/tefu ELISA Kit, dATM ELISA Kit, tef ELISA Kit, Xatm ELISA Kit, at1 ELISA Kit, atdc ELISA Kit, tel1 ELISA Kit, telo1 ELISA Kit, AT1 ELISA Kit, ATA ELISA Kit, ATC ELISA Kit, ATD ELISA Kit, ATDC ELISA Kit, ATE ELISA Kit, TEL1 ELISA Kit, TELO1 ELISA Kit, AI256621 ELISA Kit, C030026E19Rik ELISA Kit, telomere fusion ELISA Kit, ATM serine/threonine kinase L homeolog ELISA Kit, ATM serine/threonine kinase ELISA Kit, ataxia telangiectasia mutated ELISA Kit, ataxia telangiectasia mutated (atm) ELISA Kit, serine/threonine-protein kinase ATM ELISA Kit, tefu ELISA Kit, atm.L ELISA Kit, atm ELISA Kit, ATM ELISA Kit, EDI_100660 ELISA Kit, CpipJ_CPIJ001772 ELISA Kit, BDBG_08252 ELISA Kit, PAAG_02532 ELISA Kit, MCYG_05088 ELISA Kit, VDBG_06833 ELISA Kit, ACLA_015700 ELISA Kit, LOC5565620 ELISA Kit, MGYG_07634 ELISA Kit, PGTG_14279 ELISA Kit, Atm ELISA Kit
- 背景
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Gene Name: Ataxia Telangiectasia Mutated
Gene Aliases: AT1, ATD, ATA, ATC, ATDC, ATE, TEL1, TELO1, Serine-protein kinase ATM
- 基因ID
- 472
- UniProt
- Q13315
- 途径
- p53 Pathway, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
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