ACVR1 ELISA 试剂盒
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- 抗原 See all ACVR1 (ACRV1) ELISA试剂盒
- ACVR1 (ACRV1) (Activin Receptor Type I (ACRV1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Activin A Receptor Type I (ACVR1). No significant cross-reactivity or interference between Activin A Receptor Type I (ACVR1) and analogues was observed.
- 灵敏度
- 0.063 ng/mL
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Activin Receptor Type I (ACRV1) ELISA Kit
ACRV1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin A Receptor Type I (ACVR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin A Receptor Type I (ACVR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin A Receptor Type I (ACVR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activin A Receptor Type I (ACVR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A Receptor Type I (ACVR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A Receptor Type I (ACVR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ACVR1 (ACRV1) ELISA试剂盒
- ACVR1 (ACRV1) (Activin Receptor Type I (ACRV1))
- 别名
- Activin A Receptor Type I (ACRV1 产品)
- 别名
- ACTRI ELISA Kit, ACVR1A ELISA Kit, ACVRLK2 ELISA Kit, ALK2 ELISA Kit, FOP ELISA Kit, SKR1 ELISA Kit, TSRI ELISA Kit, ActR-IA ELISA Kit, ActR-I ELISA Kit, ActRIA ELISA Kit, Acvr ELISA Kit, Acvrlk2 ELISA Kit, Alk-2 ELISA Kit, Alk8 ELISA Kit, D330013D15Rik ELISA Kit, Tsk7L ELISA Kit, actri ELISA Kit, acvr1 ELISA Kit, acvr1-a ELISA Kit, acvr1-b ELISA Kit, acvr1a ELISA Kit, acvrlk2 ELISA Kit, alk-2 ELISA Kit, alk2 ELISA Kit, alk8 ELISA Kit, fop ELISA Kit, sax ELISA Kit, skr1 ELISA Kit, tsri ELISA Kit, xALK-2 ELISA Kit, activin A receptor type 1 ELISA Kit, activin A receptor, type 1 ELISA Kit, activin A receptor type 1 S homeolog ELISA Kit, ACVR1 ELISA Kit, Acvr1 ELISA Kit, acvr1.S ELISA Kit
- 背景
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Gene Name: Activin A Receptor Type I
Gene Aliases: ACVR1A, ALK2, ACVRLK2, TSRI, FOP, SKR1, ACTRI, Activin receptor-like kinase 2, Serine/threonine-protein kinase receptor R1, TGF-B superfamily receptor type I
- 基因ID
- 90
- UniProt
- Q04771
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