ATG12 ELISA 试剂盒
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- 抗原 See all ATG12 ELISA试剂盒
- ATG12 (Autophagy Related 12 (ATG12))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ATG12 in human tissue homogenates, cell lysates and other biological fluids.
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of this index.
- 灵敏度
- 0.057 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate
- Standard (freeze dried)
- Standard Diluent
- Detection Reagent A
- Detection Reagent B
- Assay Diluent A
- Assay Diluent B
- TMB
- Stop Solution
- Wash Buffer (30X)
- Plate sealer for 96 wells
- Instruction manual
- 试剂未包括
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- Microplate reader with 450 ± 10nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution.
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Autophagy Related 12 (ATG12) ELISA Kit
ATG12 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Autophagy Related 12 (ATG12) ELISA KitATG12 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Autophagy Related 12 (ATG12) ELISA KitATG12 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. - 实验流程
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The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
- CV(%) = SD/meanX100
- Intra-assay: CV<10%
- Inter-assay: CV<12%
- 限制
- 仅限研究用
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- 注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- 注意事项
- The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
- 有效期
- 12 months
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- 抗原 See all ATG12 ELISA试剂盒
- ATG12 (Autophagy Related 12 (ATG12))
- 别名
- Autophagy Related Protein 12 (ATG12) (ATG12 产品)
- 别名
- APG12 ELISA Kit, APG12L ELISA Kit, FBR93 ELISA Kit, HAPG12 ELISA Kit, Apg12l ELISA Kit, AUTOPHAGY 12 ELISA Kit, AUTOPHAGY 12 B ELISA Kit, CG10861 ELISA Kit, Dmel\\CG10861 ELISA Kit, atg12 ELISA Kit, ATG12 ELISA Kit, apg12 ELISA Kit, DDBDRAFT_0185323 ELISA Kit, DDBDRAFT_0191412 ELISA Kit, DDB_0185323 ELISA Kit, DDB_0191412 ELISA Kit, 4931423H11Rik ELISA Kit, A330058M13Rik ELISA Kit, Atg12l ELISA Kit, AO090001000593 ELISA Kit, autophagy related 12 ELISA Kit, Ubiquitin-like superfamily protein ELISA Kit, Atg12p ELISA Kit, autophagy protein ELISA Kit, ubiquitin-like protein ATG12B ELISA Kit, autophagy associated protein Atg12 ELISA Kit, Autophagy-related 12 ELISA Kit, ATG12 autophagy related 12 homolog (S. cerevisiae) ELISA Kit, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene ELISA Kit, autophagy associated ubiquitin-like modifier Atg12 ELISA Kit, hypothetical protein ELISA Kit, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) ELISA Kit, autophagy protein 12 ELISA Kit, autophagy related 12 S homeolog ELISA Kit, autophagy-related protein 12 ELISA Kit, ATG12 ELISA Kit, Atg12 ELISA Kit, APG12B ELISA Kit, APG12 ELISA Kit, LOC9321066 ELISA Kit, apg12 ELISA Kit, atg12 ELISA Kit, LOC743425 ELISA Kit, CAALFM_C113950CA ELISA Kit, atg12.S ELISA Kit, ANI_1_922094 ELISA Kit, AOR_1_1064164 ELISA Kit
- 背景
- Alternative name: APG12, APG12L, HAPG12, Ubiquitin-like protein ATG12
- 基因ID
- 9140
- UniProt
- O94817
- 途径
- Autophagy
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