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ATG12 ELISA 试剂盒

This Colorimetric ELISA kit is designed for the quantitative measurement of 小鼠 ATG12.
产品编号 ABIN8075105
发货至: 中国
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Quick Overview for ATG12 ELISA 试剂盒 (ABIN8075105)

抗原

See all ATG12 ELISA试剂盒
ATG12 (Autophagy Related 12 (ATG12))

适用

  • 6
  • 3
  • 3
  • 1
小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Lysate, Tissue Homogenate
  • 最低检测浓度

    31.2 pg/mL

    原理

    Mouse Autophagy Related Protein 12 (ATG12) ELISA Kit for detecting ATG12 in tissue homogenates, cell lysates

    Analytical Method

    Quantitative

    灵敏度

    12.1 pg/mL

    组件

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    试剂未包括

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    样本量

    100 μL

    实验时间

    3 h

    板类型

    Pre-coated

    实验精密度

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    4°C for one month, Long term: see manual.

    有效期

    12 months
  • 抗原 See all ATG12 ELISA试剂盒

    ATG12 (Autophagy Related 12 (ATG12))

    别名

    Autophagy Related Protein 12

    背景

    Gene Aliases: APG12, APG12L, HAPG12, Ubiquitin-like protein ATG12

    基因ID

    67526

    UniProt

    Q9CQY1

    途径

    Autophagy
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