ATG12 ELISA 试剂盒
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- 抗原 See all ATG12 ELISA试剂盒
- ATG12 (Autophagy Related 12 (ATG12))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ATG12 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product ATG12 ELISA Kit
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Autophagy Related 12 (ATG12) ELISA Kit
ATG12 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Autophagy Related 12 (ATG12) ELISA KitATG12 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Autophagy Related 12 (ATG12) ELISA KitATG12 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all ATG12 ELISA试剂盒
- ATG12 (Autophagy Related 12 (ATG12))
- 别名
- ATG12 (ATG12 产品)
- 别名
- APG12 ELISA Kit, APG12L ELISA Kit, FBR93 ELISA Kit, HAPG12 ELISA Kit, Apg12l ELISA Kit, AUTOPHAGY 12 ELISA Kit, AUTOPHAGY 12 B ELISA Kit, CG10861 ELISA Kit, Dmel\\CG10861 ELISA Kit, atg12 ELISA Kit, ATG12 ELISA Kit, apg12 ELISA Kit, DDBDRAFT_0185323 ELISA Kit, DDBDRAFT_0191412 ELISA Kit, DDB_0185323 ELISA Kit, DDB_0191412 ELISA Kit, 4931423H11Rik ELISA Kit, A330058M13Rik ELISA Kit, Atg12l ELISA Kit, AO090001000593 ELISA Kit, autophagy related 12 ELISA Kit, Ubiquitin-like superfamily protein ELISA Kit, Atg12p ELISA Kit, autophagy protein ELISA Kit, ubiquitin-like protein ATG12B ELISA Kit, autophagy associated protein Atg12 ELISA Kit, Autophagy-related 12 ELISA Kit, ATG12 autophagy related 12 homolog (S. cerevisiae) ELISA Kit, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene ELISA Kit, autophagy associated ubiquitin-like modifier Atg12 ELISA Kit, hypothetical protein ELISA Kit, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) ELISA Kit, autophagy protein 12 ELISA Kit, autophagy related 12 S homeolog ELISA Kit, autophagy-related protein 12 ELISA Kit, ATG12 ELISA Kit, Atg12 ELISA Kit, APG12B ELISA Kit, APG12 ELISA Kit, LOC9321066 ELISA Kit, apg12 ELISA Kit, atg12 ELISA Kit, LOC743425 ELISA Kit, CAALFM_C113950CA ELISA Kit, atg12.S ELISA Kit, ANI_1_922094 ELISA Kit, AOR_1_1064164 ELISA Kit
- 基因ID
- 9140
- UniProt
- O94817
- 途径
- Autophagy
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