PAK2 ELISA 试剂盒
PAK2
适用: 人
pSer20
Colorimetric
Cell ELISA
Adherent Cell Culture
-
- 抗原 See all PAK2 ELISA试剂盒
- PAK2 (P21-Activated Kinase 2 (PAK2))
- 抗原表位
- pSer20, total
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Cell ELISA
- 应用范围
- ELISA
- 原理
- Cell-Based ELISA Kit. This assay semi-quantitatively measures PAK2 phosphorylated at Serine-20 as well as total PAK2 in adherent cell lines.
- 样品类型
- Adherent Cell Culture
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human PAK2 phosphorylated at site Serine-20 as well as total PAK2.
- 产品特性
-
- Rapidly measure phosphorylated protein in adherent cell lines
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- No sample lysis is needed
- Compatible with a standard ELISA plate reader
- 组件
-
- Uncoated 96-well Strip Microplate
- Wash Buffers
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
- 试剂未包括
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product PAK2 ELISA Kit
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all PAK2 ELISA试剂盒
- PAK2 (P21-Activated Kinase 2 (PAK2))
- 别名
- PAK2 (PAK2 产品)
- 别名
- PAK65 ELISA Kit, PAKgamma ELISA Kit, gamma-PAK ELISA Kit, 5330420P17Rik ELISA Kit, A130002K10Rik ELISA Kit, AI836325 ELISA Kit, D16Ertd269e ELISA Kit, PAK-2 ELISA Kit, mKIAA4182 ELISA Kit, pak2 ELISA Kit, cb422 ELISA Kit, kinase ELISA Kit, zgc:91798 ELISA Kit, wu:fb71h01 ELISA Kit, si:dz198m22.1 ELISA Kit, pak65 ELISA Kit, pakgamma ELISA Kit, PAKI ELISA Kit, Pak3 ELISA Kit, CH73-159L23.1 ELISA Kit, wu:fc52b03 ELISA Kit, zgc:113857 ELISA Kit, p21 (RAC1) activated kinase 2 ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2 ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2a ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2 S homeolog ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2b ELISA Kit, PAK2 ELISA Kit, Pak2 ELISA Kit, pak2a ELISA Kit, pak2.S ELISA Kit, pak2b ELISA Kit
- 基因ID
- 5062
- UniProt
- Q13177
- 途径
- MAPK Pathway, RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
-
You are here:
