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PAK2 ELISA 试剂盒

PAK2 适用: 人 pSer20 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
产品编号 ABIN4886234
发货至: 中国
  • 抗原 See all PAK2 ELISA试剂盒
    PAK2 (P21-Activated Kinase 2 (PAK2))
    抗原表位
    pSer20, total
    适用
    • 7
    • 4
    • 4
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Phospho-PAK2 (Ser20) and Total PAK2 ELISA Kit. This assay semi-quantitatively measures phophorylated PAK2 (Ser20) and Total PAK2 in lysate samples.
    样品类型
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    特异性
    This ELISA kit recognizes Human PAK2 phosphorylated at site Serine-20 as well as total PAK2.
    产品特性
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product PAK2 ELISA Kit
  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all PAK2 ELISA试剂盒
    PAK2 (P21-Activated Kinase 2 (PAK2))
    别名
    PAK2 (PAK2 产品)
    别名
    PAK65 ELISA Kit, PAKgamma ELISA Kit, gamma-PAK ELISA Kit, 5330420P17Rik ELISA Kit, A130002K10Rik ELISA Kit, AI836325 ELISA Kit, D16Ertd269e ELISA Kit, PAK-2 ELISA Kit, mKIAA4182 ELISA Kit, pak2 ELISA Kit, cb422 ELISA Kit, kinase ELISA Kit, zgc:91798 ELISA Kit, wu:fb71h01 ELISA Kit, si:dz198m22.1 ELISA Kit, pak65 ELISA Kit, pakgamma ELISA Kit, PAKI ELISA Kit, Pak3 ELISA Kit, CH73-159L23.1 ELISA Kit, wu:fc52b03 ELISA Kit, zgc:113857 ELISA Kit, p21 (RAC1) activated kinase 2 ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2 ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2a ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2 S homeolog ELISA Kit, p21 protein (Cdc42/Rac)-activated kinase 2b ELISA Kit, PAK2 ELISA Kit, Pak2 ELISA Kit, pak2a ELISA Kit, pak2.S ELISA Kit, pak2b ELISA Kit
    基因ID
    5062
    UniProt
    Q13177
    途径
    MAPK Pathway, RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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