Glutathione Peroxidase 1 ELISA 试剂盒
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- 抗原 See all Glutathione Peroxidase 1 (GPX1) ELISA试剂盒
- Glutathione Peroxidase 1 (GPX1)
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- 18.75 pg/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
- Featured
- Discover our best selling GPX1 ELISA Kit
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- Discover our top product GPX1 ELISA Kit
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Glutathione Peroxidase 1 (GPX1) ELISA Kit
GPX1 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 适用: 小鼠 Colorimetric Sandwich ELISA 4.688 IU/mL - 300 IU/mL Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 适用: 马 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 适用: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glutathione Peroxidase 1 (GPX1) ELISA KitGPX1 适用: Cow Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
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- 样本量
- 100 µL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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- Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
- Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
- 试剂准备
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- Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
- Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
- Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 2000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 2000, 1000, 500, 250, 125, 62.5, 31.25, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 2000 pg/mL working solution to the first tube and mix up to produce a 1000 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
- Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
- Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
-
- 抗原 See all Glutathione Peroxidase 1 (GPX1) ELISA试剂盒
- Glutathione Peroxidase 1 (GPX1)
- 别名
- GPX1(Glutathione Peroxidase 1) (GPX1 产品)
- 别名
- Gpx ELISA Kit, ATGPX1 ELISA Kit, F13D4.40 ELISA Kit, F13D4_40 ELISA Kit, GLUTATHIONE PEROXIDASE 1 ELISA Kit, glutathione peroxidase 1 ELISA Kit, gshpx1 ELISA Kit, AI195024 ELISA Kit, AL033363 ELISA Kit, CGPx ELISA Kit, GPx-1 ELISA Kit, GSHPx-1 ELISA Kit, GPX1 ELISA Kit, gpx1 ELISA Kit, cb690 ELISA Kit, zgc:92737 ELISA Kit, zgc:103683 ELISA Kit, GPXD ELISA Kit, GSHPX1 ELISA Kit, GSHPx ELISA Kit, wu:fd42f12 ELISA Kit, wu:fj22d06 ELISA Kit, zgc:101700 ELISA Kit, glutathione peroxidase Gpx1 ELISA Kit, glutathione peroxidase ELISA Kit, glutathione peroxidase 1 ELISA Kit, glutathione peroxidase 1a ELISA Kit, glutathione peroxidase family protein ELISA Kit, proliferating cell nuclear antigen ELISA Kit, glutathione peroxidase 1b ELISA Kit, glutathione peroxidase 1 S homeolog ELISA Kit, gpx1 ELISA Kit, GPX1 ELISA Kit, gpx ELISA Kit, Gpx ELISA Kit, Gpx1 ELISA Kit, gpx1a ELISA Kit, POPTR_0003s12620g ELISA Kit, Pcna ELISA Kit, LBL_RS00590 ELISA Kit, btuE-1 ELISA Kit, GPX2 ELISA Kit, gpx1b ELISA Kit, gpx1.S ELISA Kit
- 途径
- Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome
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