HMGCR ELISA 试剂盒
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- 抗原 See all HMGCR ELISA试剂盒
- HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.63 ng/mL - 40 ng/mL
- 最低检测浓度
- 0.63 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- 0.38 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
- Featured
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- Discover our top product HMGCR ELISA Kit
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3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) ELISA Kit
HMGCR 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) ELISA KitHMGCR 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) ELISA KitHMGCR 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) ELISA KitHMGCR 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) ELISA KitHMGCR 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) ELISA KitHMGCR 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 µL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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- Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
- Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
- 试剂准备
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- Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
- Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
- Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 40 ng/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 40, 20, 10, 5, 2.5, 1.25, 0.63, 0 ng/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 40 ng/mL working solution to the first tube and mix up to produce a 20 ng/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
- Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
- Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
-
- 抗原 See all HMGCR ELISA试剂盒
- HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))
- 别名
- HMGCR(3-Hydroxy-3-methylglutaryl CoA Reductase) (HMGCR 产品)
- 别名
- HMGR ELISA Kit, LDLCQ3 ELISA Kit, hmg-coa-r ELISA Kit, HMG-CoAR ELISA Kit, Red ELISA Kit, 3H3M ELISA Kit, BcDNA:LD15354 ELISA Kit, CG10367 ELISA Kit, Clb ELISA Kit, DmHMG-CoAr ELISA Kit, Dmel\\CG10367 ELISA Kit, HMGCR ELISA Kit, HMGCR_Dm ELISA Kit, HMGCoAR ELISA Kit, HMGCoARr ELISA Kit, HMGCoAr ELISA Kit, HmG-CoAR ELISA Kit, Hmg ELISA Kit, bro1 ELISA Kit, bs32f05.y1 ELISA Kit, clb ELISA Kit, hmgc ELISA Kit, hmgcr ELISA Kit, hmgrc ELISA Kit, l(3)01152 ELISA Kit, l(3)04684 ELISA Kit, hmgcr2 ELISA Kit, im:6912716 ELISA Kit, zgc:110746 ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase L homeolog ELISA Kit, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ELISA Kit, HMG Coenzyme A reductase ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase b ELISA Kit, HMGCR ELISA Kit, hmgcr.L ELISA Kit, Hmgcr ELISA Kit, hmgcrb ELISA Kit
- 途径
- AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha
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