Lipoxin B4 ELISA 试剂盒
适用: Various Species
Colorimetric
Competition ELISA
0.313-20 ng/mL
Plasma, Serum
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- 抗原
- Lipoxin B4
- 适用
- Various Species
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 0.313-20 ng/mL
- 最低检测浓度
- 0.313 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- 0.188 ng/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
- Featured
- Discover our best selling ELISA Kit
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Lipoxin B4 ELISA Kit
适用: Various Species Colorimetric Competition ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Lipoxin B4 ELISA Kit适用: Various Species Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 50µL
- 板类型
- Pre-coated
- 实验流程
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- Add 50 µL standard or sample to each well. Immediately add 50 µL Biotinylated Detection Antibody to each well. Incubate for 45 min at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate to each well. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
- 试剂准备
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- Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
- Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer. Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved.
- Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 20 ng/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 20, 10, 5, 2.5, 1.25, 0.63, 0.31, 0 ng/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 20 ng/mL stock solution to the first tube and mix up to produce a 10 ng/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
- Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (50 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
- Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
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- 抗原
- Lipoxin B4
- 别名
- LXB4(Lipoxin B4)
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