ADIPOQ ELISA 试剂盒
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- 抗原 See all ADIPOQ ELISA试剂盒
- ADIPOQ (Adiponectin (ADIPOQ))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- This ELISA is a sandwich enzyme immunoassay for the quantitative measurement of mouse Adiponectin/Acrp30 protein in serum. It is intended for in vitro research use only.
- 样品类型
- Serum
- Analytical Method
- Quantitative
- 灵敏度
- 0.1 ng/mL
- 产品特性
- Adiponectin, Mouse ELISA Kit
- Featured
- Discover our best selling ADIPOQ ELISA Kit
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- Discover our top product ADIPOQ ELISA Kit
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Adiponectin (ADIPOQ) ELISA Kit
ADIPOQ 适用: 人 Colorimetric Sandwich ELISA 1.562-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 绵羊 Colorimetric Competition ELISA 6.25 ng/mL - 100 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鸡 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 小鼠 AA 18-247 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
Adiponectin (ADIPOQ) ELISA KitADIPOQ 适用: 人 AA 19-244 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
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- 实验流程
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Principle:
- The surface of the wells in a microtitration plate is coated with monoclonal anti-mouse Adiponectin specific antibody. The standards, quality controls (QC), and diluted samples are pipetted into the wells. Any mouse Adiponectin/Acrp30 present is captured by immobilized antibody and unbound protein is washed away after the first incubation period. Then, a horseradish peroxidase (HRP) conjugated polyclonal anti-mouse Adiponectin antibody is added to the wells and incubated. Following another washing step, to remove unbound antibody-HRP conjugate, a substrate solution (H2O2 and TMB) is added to the wells. The enzymatic reaction yields a blue product that turns yellow when acidic stop solution is added. The intensity of the color, measured spectrophotochemically at 450nm, is directly proportional to the amount of mouse Adiponectin bound in the initial step. The concentrations of the diluted test samples are then read off the standard curve that is constructed by plotting the absorbance values against each respective mouse Adiponectin standard level using a four-parameter function. The dilution factor needs to be taken into consideration when calculating the actual concentration of Adiponectin/Acrp30 analyte in the test samples.
- 实验精密度
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Precision pipettes to deliver 10-1000ul and disposable tips
- 3. Multichannel pipette 50-200ul
- 4. Microplate reader with 450 ± 10nm filter
- 5. Orbital microplate shaker capable of agitation at approximately 300rpm
- 6. Software package facilitating data generation and analysis
- 7. Microplate washer (optional). Manual washing is possible but not preferable.
- 8. Glassware (graduated cylinder and bottle for Wash Solution)
- 9. Distilled water
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-80 °C
- 储存方法
- 4°C/-70°C
-
- 抗原 See all ADIPOQ ELISA试剂盒
- ADIPOQ (Adiponectin (ADIPOQ))
- 别名
- Adiponectin (ADIPOQ 产品)
- 别名
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