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PDGF-BB Homodimer ELISA 试剂盒

适用: 小鼠 Colorimetric Sandwich ELISA Plasma, Serum, Tissue Homogenate
产品编号 ABIN5067997
发货至: 中国
  • 抗原
    PDGF-BB Homodimer
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    The Mouse Platelet-Derived Growth Factor-BB (PDGF-BB) ELISA Kit is a quantitative sandwich assay for the detection of PDGF-BB in mouse serum, plasma, tissue homogenates and other biological fluids.
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    23.438 pg/mL
    产品特性
    PDGF-BB (Platelet-Derived Growth Factor-BB) ELISA Kit (Mouse)
    组件
    • *357966A: Microtiter Strips, 8x12 wells.
    • *357966B: Standard, 2 vials
    • 357966C: Sample/Standard Dilution Buffer, 1x20ml
    • 357966D: Antibody-Biotin (Concentrated), 1x120ul
    • 357966E: Antibody Dilution Buffer, 1x10ml
    • 357966F: Streptavidin-HRP Conjugate (SABC), 1x120ul
    • 357966G: SABC Dilution Buffer, 1x10ml
    • 357966H: TMB Substrate, 1x10ml
    • 357966J: Stop Solution, 1x10ml
    • 357966K: Wash Buffer (25X), 1x30ml
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  • 板类型
    Pre-coated
    实验流程
    Principle:
    • The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGF-BB. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to PDGF-BB. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDGF-BB, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDGF-BB in the sample is then determined by comparing the O.D. of the sample to the standard curve.
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    4°C/-20°C
  • 抗原
    PDGF-BB Homodimer
    别名
    PDGF-BB (Platelet-Derived Growth Factor-BB)
    别名
    PDGF-2 ELISA Kit, PDGF2 ELISA Kit, SIS ELISA Kit, SSV ELISA Kit, c-sis ELISA Kit, PDGF-B ELISA Kit, Sis ELISA Kit, platelet derived growth factor subunit B ELISA Kit, platelet derived growth factor, B polypeptide ELISA Kit, PDGFB ELISA Kit, Pdgfb ELISA Kit
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