Bcl-2 ELISA 试剂盒
Quick Overview for Bcl-2 ELISA 试剂盒 (ABIN5026957)
抗原
See all Bcl-2 (BCL2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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灵敏度
- < 0.094 ng/mL
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组件
- plate, standard, diluent, antibodies
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试剂未包括
- pipettes, tubes, reader, incubator
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B-Cell CLL/lymphoma 2 (BCL2) ELISA Kit
BCL2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
B-Cell CLL/lymphoma 2 (BCL2) ELISA KitBCL2 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
B-Cell CLL/lymphoma 2 (BCL2) ELISA KitBCL2 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
B-Cell CLL/lymphoma 2 (BCL2) ELISA KitBCL2 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
B-Cell CLL/lymphoma 2 (BCL2) ELISA KitBCL2 适用: 豚鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
B-Cell CLL/lymphoma 2 (BCL2) ELISA KitBCL2 适用: 兔 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human Bcl-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human Bcl-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Bcl-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human Bcl-2 can be calculated using the standard curve.
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限制
- 仅限研究用
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注意事项
- protect your eyes
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储存条件
- 4 °C
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- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
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别名
- Bcl -2 (B cell Lymphoma 2)
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途径
- MAPK Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
抗原 See all Bcl-2 (BCL2) ELISA试剂盒
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