Tumor Necrosis Factor a ELISA 试剂盒
适用: 人
Colorimetric
Sandwich ELISA
15.6-1000 pg/mL
Plasma, Serum
1 image
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- 抗原
- Tumor Necrosis Factor a
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6-1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of Human TNFBeta concentrations in serum, plasma and other biological fluids.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- < 9.4 pg/mL
- 组件
- plate, standard, diluent, antibodies
- 试剂未包括
- pipettes, tubes, reader, incubator
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- 样本量
- 100 μL
- 实验时间
- 3 - 4 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs an antibody specific for Anti-human TNFBeta coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFBeta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNFBeta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFBeta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- 限制
- 仅限研究用
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- 注意事项
- protect your eyes
- 储存条件
- 4 °C
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- 抗原
- Tumor Necrosis Factor a
- 别名
- TNFalpha (Tumor Necrosis Factor Beta)
- 别名
- DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit, TNFa ELISA Kit, tumor necrosis factor ELISA Kit, TNF ELISA Kit
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