PD-1 ELISA 试剂盒
Quick Overview for PD-1 ELISA 试剂盒 (ABIN5026948)
抗原
See all PD-1 (PDCD1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This immunoassay kit allows for the in vitro quantitative determination of Human PDL1 concentrations in serum, plasma and other biological fluids. This kit is for Research use only. Not for use in diagnostic/therapeutics procedures.
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Analytical Method
- Quantitative
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灵敏度
- < 0.094 ng/mL
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组件
- plate, standard, diluent, antibodies
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试剂未包括
- pipettes, tubes, reader, incubator
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Programmed Cell Death 1 (PDCD1) ELISA Kit
PDCD1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: Various Species Colorimetric Competition ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 AA 25-167 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant
Programmed Cell Death 1 (PDCD1) ELISA KitPDCD1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human PD-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human PD-1 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PD-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human PD-1 can be calculated using the standard curve.
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限制
- 仅限研究用
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注意事项
- protect your eyes
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储存条件
- 4 °C
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- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
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别名
- PD-1 (Programmed Cell Death Protein1)
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途径
- Cancer Immune Checkpoints
抗原 See all PD-1 (PDCD1) ELISA试剂盒
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