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- 抗原 See all FNDC5 ELISA试剂盒
- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156-10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FNDC5 in human serum, plasma and other biological fluids
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This kit recognizes natural and recombinantHumanFNDC5. No significant cross-reactivity or interference between HumanFNDC5 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between HumanFNDC5 and all the analogues.
- 灵敏度
- 0.094 ng/mL
- 组件
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Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis - 试剂未包括
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Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer - Featured
- Discover our best selling FNDC5 ELISA Kit
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- Discover our top product FNDC5 ELISA Kit
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Fibronectin Type III Domain Containing 5 (FNDC5) ELISA Kit
FNDC5 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fibronectin Type III Domain Containing 5 (FNDC5) ELISA KitFNDC5 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fibronectin Type III Domain Containing 5 (FNDC5) ELISA KitFNDC5 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Fibronectin Type III Domain Containing 5 (FNDC5) ELISA KitFNDC5 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Fibronectin Type III Domain Containing 5 (FNDC5) ELISA KitFNDC5 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Fibronectin Type III Domain Containing 5 (FNDC5) ELISA KitFNDC5 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fibronectin Type III Domain Containing 5 (FNDC5) ELISA KitFNDC5 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fibronectin Type III Domain Containing 5 (FNDC5) ELISA KitFNDC5 适用: 马 Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Fibronectin Type III Domain Containing 5 (FNDC5) ELISA KitFNDC5 适用: Cow Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Fibronectin Type III Domain Containing 5 (FNDC5) ELISA KitFNDC5 适用: 人 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).
Information on reagents:
Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.
Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. - 样本量
- 100 µL
- 板类型
- Pre-coated
- 实验流程
- This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FNDC5. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FNDC5and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FNDC5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FNDC5.You can calculate the concentration of FNDC5in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
- 限制
- 仅限研究用
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- 注意事项
- All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
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- 抗原 See all FNDC5 ELISA试剂盒
- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
- 别名
- Fibronectin type III domain-containing protein 5 (FNDC5) (FNDC5 产品)
- 别名
- FRCP2 ELISA Kit, irisin ELISA Kit, 1500001L03Rik ELISA Kit, AI836596 ELISA Kit, C87088 ELISA Kit, PeP ELISA Kit, Pxp ELISA Kit, fndc5 ELISA Kit, si:dkey-264p5.1 ELISA Kit, zgc:158684 ELISA Kit, fibronectin type III domain containing 5 ELISA Kit, fibronectin type III domain containing 5b ELISA Kit, FNDC5 ELISA Kit, Fndc5 ELISA Kit, fndc5b ELISA Kit
- 途径
- Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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