Dihydrofolate Reductase ELISA 试剂盒
-
- 抗原 See all Dihydrofolate Reductase (DHFR) ELISA试剂盒
- Dihydrofolate Reductase (DHFR)
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78.125-5000 pg/mL
- 最低检测浓度
- 78.125 pg/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DHFR in human serum, plasma and other biological fluids
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This kit recognizes natural and recombinantHumanDHFR. No significant cross-reactivity or interference between HumanDHFR and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between HumanDHFR and all the analogues.
- 灵敏度
- 46.875 pg/mL
- 组件
-
Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis - 试剂未包括
-
Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer - Featured
- Discover our best selling DHFR ELISA Kit
- Top Product
- Discover our top product DHFR ELISA Kit
-
Dihydrofolate Reductase (DHFR) ELISA Kit
DHFR 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Dihydrofolate Reductase (DHFR) ELISA KitDHFR 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Dihydrofolate Reductase (DHFR) ELISA KitDHFR 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
-
- 说明
-
Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).
Information on reagents:
Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.
Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. - 样本量
- 100 µL
- 板类型
- Pre-coated
- 实验流程
- This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DHFR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DHFRand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DHFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of DHFR.You can calculate the concentration of DHFRin the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
- 限制
- 仅限研究用
-
- 注意事项
- All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
-
- 抗原 See all Dihydrofolate Reductase (DHFR) ELISA试剂盒
- Dihydrofolate Reductase (DHFR)
- Abstract
- DHFR 产品
- 别名
- DHFR ELISA Kit, F10_orf160 ELISA Kit, ECK0049 ELISA Kit, JW0047 ELISA Kit, tmrA ELISA Kit, cb595 ELISA Kit, zgc:86637 ELISA Kit, DDBDRAFT_0187132 ELISA Kit, DDBDRAFT_0230113 ELISA Kit, DDB_0187132 ELISA Kit, DDB_0230113 ELISA Kit, CG14887 ELISA Kit, Dmel\\CG14887 ELISA Kit, dhfr ELISA Kit, DHFRP1 ELISA Kit, DYR ELISA Kit, 8430436I03Rik ELISA Kit, AA607882 ELISA Kit, AI662710 ELISA Kit, AW555094 ELISA Kit, Dhfr1 ELISA Kit, confers resistance to sulfonamides ELISA Kit, dihydrofolate reductase ELISA Kit, dihydrofolate reductase DhfR ELISA Kit, Dihydrofolate reductase ELISA Kit, dhfr ELISA Kit, DHFR ELISA Kit, dhfR ELISA Kit, folA ELISA Kit, dfrA ELISA Kit, LOC707382 ELISA Kit, CNJ01940 ELISA Kit, DDB_G0286755 ELISA Kit, Dhfr ELISA Kit, Mesil_0096 ELISA Kit, Arch_0408 ELISA Kit, Fbal_3367 ELISA Kit, Ilyop_0482 ELISA Kit, Lbys_2007 ELISA Kit, Riean_0733 ELISA Kit, Mrub_1855 ELISA Kit, Poras_0766 ELISA Kit, Halhy_3552 ELISA Kit, Mesop_3614 ELISA Kit, LOC609048 ELISA Kit
- 途径
- Mitotic G1-G1/S Phases
-
