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CD40 Ligand ELISA 试剂盒

CD40LG 适用: 人 Colorimetric Sandwich ELISA 156.25-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
产品编号 ABIN4987074
发货至: 中国
  • 抗原 See all CD40 Ligand (CD40LG) ELISA试剂盒
    CD40 Ligand (CD40LG)
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156.25-10000 pg/mL
    最低检测浓度
    156.25 pg/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Natural and recombinant Human sCD40L Ligand
    灵敏度
    40 pg/mL
    试剂未包括
    • Microplate reader.
    • Pipettes and pipette tips.
    • EP tube Deionized or distilled water.
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  • 应用备注
    Detection Wavelength: 450 nm
    样本量
    20 μL
    实验时间
    3 h
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    4 °C
  • 抗原 See all CD40 Ligand (CD40LG) ELISA试剂盒
    CD40 Ligand (CD40LG)
    别名
    sCD40L (CD40LG 产品)
    别名
    CD154 ELISA Kit, cd40l ELISA Kit, CD40L ELISA Kit, HIGM1 ELISA Kit, IGM ELISA Kit, IMD3 ELISA Kit, T-BAM ELISA Kit, TNFSF5 ELISA Kit, TRAP ELISA Kit, gp39 ELISA Kit, hCD40L ELISA Kit, CD40-L ELISA Kit, Cd40l ELISA Kit, Ly-62 ELISA Kit, Ly62 ELISA Kit, Tnfsf5 ELISA Kit, CD40 ligand ELISA Kit, TNF superfamily member 5 ELISA Kit, CD40LG ELISA Kit, cd40l ELISA Kit, Cd40lg ELISA Kit
    背景
    CD40 Ligand (CD40L), also known as CD154, gp39, TNFSF5, TRAP (TNF-Related Activation Protein) or TBAM (T-cell B-cell Activating Molecule), is a multifunctional ligand in the TNF superfamily (1-4). Interaction between CD40 and CD40L is critical to the control of thymus-dependent humoral immunity and cell-mediated immune responses (5-10). The major component of the contact-dependent signal leading to B cell activation is CD40L. CD40L stimulates B cell secretion of immunoglobulin isotypes in the presence of cytokines.CD40L is a 39 kDa, 261 amino acid (aa) glycoprotein that can form homotrimers typical of other TNFSF members (1-4, 11, 12). Proteolytic cleavage can also produce 15-18 kDa soluble forms of CD40L (13, 14). Activated T cells and platelets express both a membrane-associated and a soluble form of CD40L (sCD40L) (13, 15, 16). Platelet activation during plasma and serum sample preparation can result in artificially elevated sCD40L levels (17-20). Conversely, serum samples stored above 2-8
    途径
    NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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