CD40 Ligand ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for CD40 Ligand ELISA 试剂盒 (ABIN6719970)
抗原
See all CD40 Ligand (CD40LG) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 62.5 pg/mL
-
Supplier Product No.
- koa0349
-
Supplier
- Rockland
-
原理
- Human sCD40L Sandwich ELISA Kit for Quantitative Detection
-
品牌
- AccuSignal™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: E.coli
Immunogen sequence: E108-L261 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 15 pg/mL
-
组件
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
CD40 Ligand (CD40LG) ELISA Kit
CD40LG 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 兔 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 小鼠 Colorimetric Sandwich ELISA 1.563 μg/mL - 100 μg/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
说明
-
Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CD40L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD40L standard solution and each sample be measured in duplicate.
-
实验精密度
-
Intra-Assay Precision (CV%): 7.6%, 7.4%, 5%
Inter-Assay Precision (CV%): 8.6%, 8.1%, 6.7% -
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
有效期
- 12 months
-
-
- CD40 Ligand (CD40LG)
-
别名
- sCD40L
-
背景
-
Background: CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
Gene Full Name: CD40 ligand
-
基因ID
- 959
-
NCBI登录号
- NP_000065
-
UniProt
- P29965
-
途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原 See all CD40 Ligand (CD40LG) ELISA试剂盒
-
