JNK ELISA 试剂盒
This 人, 小鼠 和 大鼠 JNK ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人, 小鼠 和 大鼠 JNK. This ELISA Kit has been cited in1 publication.
(1 reference)Quick Overview for JNK ELISA 试剂盒 (ABIN4886162)
抗原
See all JNK (MAPK8) ELISA试剂盒
JNK (MAPK8)
(Mitogen-Activated Protein Kinase 8 (MAPK8))
抗原表位
pThr183, pTyr185, total
适用
人, 小鼠, 大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Lysate, Tissue Lysate
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原理
- Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA Kit. This assay semi-quantitatively measures phophorylated JNK (Thr183/Tyr185) and Total JNK in lysate samples.
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Analytical Method
- Semi-Quantitative
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特异性
- The antibody pair provided in this kit recognizes Human/Mouse/Rat Phospho-JNK (pThr183/Tyr185) and total (pan) JNK.
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产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Mitogen-Activated Protein Kinase 8 (MAPK8) ELISA Kit
MAPK8 适用: 人, 小鼠, 大鼠 pThr183 Colorimetric Cell ELISA Cell Culture Cells
Mitogen-Activated Protein Kinase 8 (MAPK8) ELISA KitMAPK8 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
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有效期
- 6 months
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: "Protective effects of arjunolic acid against cardiac toxicity induced by oral sodium nitrite: effects on cytokine balance and apoptosis." in: Life sciences, Vol. 111, Issue 1-2, pp. 18-26, (2014) (PubMed).
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: "Protective effects of arjunolic acid against cardiac toxicity induced by oral sodium nitrite: effects on cytokine balance and apoptosis." in: Life sciences, Vol. 111, Issue 1-2, pp. 18-26, (2014) (PubMed).
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- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
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别名
- JNK
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基因ID
- 5599
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UniProt
- P45983
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途径
- MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins
抗原 See all JNK (MAPK8) ELISA试剂盒
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