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CHEK1 ELISA 试剂盒

This anti-CHEK1 antibody (ABIN4886023) is a antibody detecting CHEK1 in ELISA. Suitable for Human.
产品编号 ABIN4886023
发货至: 中国

Quick Overview for CHEK1 ELISA 试剂盒 (ABIN4886023)

抗原

See all CHEK1 ELISA试剂盒
CHEK1 (Checkpoint Kinase 1 (CHEK1))

抗原表位

pSer280, total

适用

  • 8
  • 3
  • 2
  • 1

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Lysate, Tissue Lysate
  • 原理

    Human Phospho-Chk1 (S280) and Total Chk1 ELISA Kit. This assay semi-quantitatively measures phophorylated Chk1 (Ser280) and Total Chk1 in lysate samples.

    Analytical Method

    Semi-Quantitative

    特异性

    The antibody pair provided in this kit recognizes human Chk1 phosphorylated at site Serine-280 and total Chk1

    产品特性

    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample

    试剂未包括

    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • 样本量

    100 μL

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    -20 °C

    储存方法

    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

    有效期

    6 months
  • 抗原 See all CHEK1 ELISA试剂盒

    CHEK1 (Checkpoint Kinase 1 (CHEK1))

    别名

    CHK1

    基因ID

    1925

    UniProt

    O14757

    途径

    p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair
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