TR4 ELISA 试剂盒
NR2C2
适用: 人, 小鼠
pThr69
Colorimetric
Sandwich ELISA
Cell Culture Lysate
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- 抗原 See all TR4 (NR2C2) ELISA试剂盒
- TR4 (NR2C2) (Nuclear Receptor Subfamily 2, Group C, Member 2 (NR2C2))
- 抗原表位
- pThr69, pThr71
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适用
- 人, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human and Mouse Phospho-ATF2 (Thr69/71) ELISA Kit. This assay semi-quantitatively measures phophorylated ATF2 (Thr69/71) in lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human and Mouse TAK1 phosphorylated at site Threonine-69/71.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our best selling NR2C2 ELISA Kit
- Top Product
- Discover our top product NR2C2 ELISA Kit
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Nuclear Receptor Subfamily 2, Group C, Member 2 (NR2C2) ELISA Kit
NR2C2 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all TR4 (NR2C2) ELISA试剂盒
- TR4 (NR2C2) (Nuclear Receptor Subfamily 2, Group C, Member 2 (NR2C2))
- 别名
- TAK1 (NR2C2 产品)
- 别名
- TAK1 ELISA Kit, TR2R1 ELISA Kit, TR4 ELISA Kit, hTAK1 ELISA Kit, Tr4 ELISA Kit, gb:dq017625 ELISA Kit, im:6911408 ELISA Kit, mKIAA4145 ELISA Kit, nuclear receptor subfamily 2 group C member 2 ELISA Kit, nuclear receptor subfamily 2, group C, member 2 ELISA Kit, NR2C2 ELISA Kit, Nr2c2 ELISA Kit, nr2c2 ELISA Kit
- 基因ID
- 1386
- UniProt
- P15336, P16951
- 途径
- TCR Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Toll-Like Receptors Cascades
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