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TNF alpha ELISA 试剂盒

Name: TNF alpha ELISA 试剂盒
SKU: ABIN4885660
Availability: OutOfStock

This 小鼠 TNF alpha ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 TNF alpha.

产品编号 ABIN4885660

发货至: 中国

Quick Overview for TNF alpha ELISA 试剂盒 (ABIN4885660)

抗原

See all TNF alpha ELISA试剂盒

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

94-6000 pg/mL

应用范围

ELISA

样品类型

Cell Lysate, Tissue Lysate

Discover our top product TNF alpha ELISA Kit

最低检测浓度

94 pg/mL

原理

Mouse TNF alpha ELISA Kit for Tissue Lysates, Cell Lysates samples.

Analytical Method

Quantitative

特异性

Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1alpha, IL-1beta, IL-2, IL-3, IL-3R beta, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1alpha, MIP-1gamma, MIP-2, MIP-3 beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Cell lysate buffer
Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum

Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum

Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

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TNF alpha 适用: 大鼠 AA 80-235 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha 适用: 小鼠 AA 80-235 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
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样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
抗原 See all TNF alpha ELISA试剂盒

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

TNF alpha

基因ID

21926

UniProt

P06804

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome