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CD93 ELISA 试剂盒

CD93 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN4885135
发货至: 中国
  • 抗原 See all CD93 ELISA试剂盒
    CD93
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human C1q R1 (CD93) SpeedELISA Kit.
    品牌
    SpeedELISA
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    产品特性
    • Compressed workflow with only one wash step and three incubation steps
    • 3 hours total processing time
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Streptavidin-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Capture Antibody
    • HRP Conjugated Detection Antibody
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 70 μL of the Antibody Cocktail to each well.
    3. Incubate 10 minutes at RT.
    4. Add 50 μL of sample or standard to each well.
    5. Incubate 2 h at RT.
    6. Wash Wells
    7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    8. Incubate 30 min at RT.
    9. Add 50 μL of Stop Solution to each well.
    10. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
    Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all CD93 ELISA试剂盒
    CD93
    别名
    C1q R1 (CD93 产品)
    别名
    6030404G09Rik ELISA Kit, AA145088 ELISA Kit, AA4.1 ELISA Kit, AW555904 ELISA Kit, C1qr1 ELISA Kit, C1qrp ELISA Kit, Ly68 ELISA Kit, C1QR1 ELISA Kit, C1qR(P) ELISA Kit, C1qRP ELISA Kit, CDw93 ELISA Kit, ECSM3 ELISA Kit, MXRA4 ELISA Kit, dJ737E23.1 ELISA Kit, C1qRp ELISA Kit, CD93 antigen ELISA Kit, CD93 molecule ELISA Kit, Cd93 ELISA Kit, CD93 ELISA Kit
    基因ID
    22918
    UniProt
    Q9NPY3
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