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SULT2A1 ELISA 试剂盒

SULT2A1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
产品编号 ABIN4884692
发货至: 中国
  • 抗原 See all SULT2A1 ELISA试剂盒
    SULT2A1 (Sulfotransferase Family, Cytosolic, 2A, Dehydroepiandrosterone (DHEA)-Preferring, Member 1 (SULT2A1))
    适用
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Custom Human SULT2A1 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all SULT2A1 ELISA试剂盒
    SULT2A1 (Sulfotransferase Family, Cytosolic, 2A, Dehydroepiandrosterone (DHEA)-Preferring, Member 1 (SULT2A1))
    别名
    SULT2A1 (SULT2A1 产品)
    别名
    dhea-st ELISA Kit, dheas ELISA Kit, st2 ELISA Kit, st2a1 ELISA Kit, st2a3 ELISA Kit, sta ELISA Kit, std ELISA Kit, SULT2A1 ELISA Kit, ST2A1 ELISA Kit, Std ELISA Kit, Sth1 ELISA Kit, mSTa1 ELISA Kit, STa ELISA Kit, Smp-2 ELISA Kit, St2 ELISA Kit, St2a1 ELISA Kit, Sth2 ELISA Kit, DHEA-ST ELISA Kit, DHEAS ELISA Kit, HST ELISA Kit, ST2 ELISA Kit, ST2A3 ELISA Kit, STD ELISA Kit, hSTa ELISA Kit, sulfotransferase family 2A member 1 ELISA Kit, sulfotransferase family 2A member 1 L homeolog ELISA Kit, alcohol sulfotransferase ELISA Kit, sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 1 ELISA Kit, SULT2A1 ELISA Kit, sult2a1.L ELISA Kit, PCC8801_2155 ELISA Kit, Cyan8802_2217 ELISA Kit, NAL212_1203 ELISA Kit, sult2a1 ELISA Kit, Sult2a1 ELISA Kit
    基因ID
    6822
    UniProt
    Q06520
    途径
    Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha, Monocarboxylic Acid Catabolic Process
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