SPRY1 ELISA 试剂盒
SPRY1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all SPRY1 products
- SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human SPRY1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes .
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all SPRY1 products
- SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
- 别名
- SPRY1 (SPRY1 产品)
- 别名
- hSPRY1 ELISA Kit, SPRY1 ELISA Kit, LOC100223875 ELISA Kit, hspry1 ELISA Kit, xspry1 ELISA Kit, sprouty ELISA Kit, sprouty1 ELISA Kit, sprouty-1 ELISA Kit, Xtsprouty1 ELISA Kit, spry1a ELISA Kit, spry1b ELISA Kit, sprouty RTK signaling antagonist 1 ELISA Kit, sprouty RTK signaling antagonist 1 L homeolog ELISA Kit, sprouty RTK signaling antagonist 1 S homeolog ELISA Kit, sprouty homolog 1 (Drosophila) ELISA Kit, SPRY1 ELISA Kit, Spry1 ELISA Kit, spry1 ELISA Kit, spry1.L ELISA Kit, spry1.S ELISA Kit
- 基因ID
- 10252
- UniProt
- O43609
- 途径
- EGFR Signaling Pathway, EGFR Downregulation
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