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SEMA6A ELISA 试剂盒
SEMA6A
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
SEMA6AELISA Kit详情
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SEMA6A
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6A (SEMA6A))
适用
All reactivities for SEMA6A ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human Semaphorin 6A ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes human Semaphorin 6A. Other species not yet determined.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
SEMA6A目标详情
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SEMA6A
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6A (SEMA6A))
别名
Semaphorin 6A (SEMA6A 产品 )
别名
fj46c12 ELISA Kit, wu:fj46c12 ELISA Kit, zgc:66106 ELISA Kit, SEMA6A ELISA Kit, HT018 ELISA Kit, SEMA ELISA Kit, SEMA6A1 ELISA Kit, SEMAQ ELISA Kit, VIA ELISA Kit, 9330158E07 ELISA Kit, A730020P05Rik ELISA Kit, AI851735 ELISA Kit, Sema6A-1 ELISA Kit, Semaq ELISA Kit, VIa ELISA Kit, b2b997Clo ELISA Kit, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A ELISA Kit, semaphorin 6A ELISA Kit, sema6a ELISA Kit, SEMA6A ELISA Kit, Sema6a ELISA Kit
基因ID
57556
UniProt
Q9H2E6
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