S100A11 ELISA 试剂盒
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- 抗原 See all S100A11 ELISA试剂盒
- S100A11 (S100 Calcium Binding Protein A11 (S100A11))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.034-25 ng/mL
- 最低检测浓度
- 0.034 ng/mL
- 应用范围
- ELISA
- 原理
- Human S100 A11 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human S100 A11.
- 灵敏度
- 0.03 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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S100 Calcium Binding Protein A11 (S100A11) ELISA Kit
S100A11 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A11 (S100A11) ELISA KitS100A11 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A11 (S100A11) ELISA KitS100A11 适用: Pig Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A11 (S100A11) ELISA KitS100A11 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all S100A11 ELISA试剂盒
- S100A11 (S100 Calcium Binding Protein A11 (S100A11))
- 别名
- S100 A11 (S100A11 产品)
- 别名
- MLN70 ELISA Kit, S100C ELISA Kit, PCALG ELISA Kit, EMAPI ELISA Kit, Emap1 ELISA Kit, S100a14 ELISA Kit, S100c ELISA Kit, cal ELISA Kit, S100 calcium binding protein A11 ELISA Kit, S100A11 ELISA Kit, S100a11 ELISA Kit
- 基因ID
- 6282
- UniProt
- P31949
- 途径
- S100 Proteins
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