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SPI1 ELISA 试剂盒
SPI1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
SPI1ELISA Kit详情
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SPI1
(Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
适用
All reactivities for SPI1 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human PU.1/Spi-1 ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes .
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
SPI1目标详情
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SPI1
(Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
别名
PU.1 (SPI1 产品 )
别名
OF ELISA Kit, PU.1 ELISA Kit, SFPI1 ELISA Kit, SPI-1 ELISA Kit, SPI-A ELISA Kit, Dis-1 ELISA Kit, Dis1 ELISA Kit, Sfpi-1 ELISA Kit, Sfpi1 ELISA Kit, Spi-1 ELISA Kit, Tcfpu1 ELISA Kit, Tfpu.1 ELISA Kit, Pu.1 ELISA Kit, cb1086 ELISA Kit, pu.1 ELISA Kit, pu1 ELISA Kit, sfpi1 ELISA Kit, si:by184l24.2 ELISA Kit, spi1 ELISA Kit, SPI1/PU.1 ELISA Kit, spi1l ELISA Kit, zgc:152991 ELISA Kit, Spi-1 proto-oncogene ELISA Kit, spleen focus forming virus (SFFV) proviral integration oncogene ELISA Kit, Spi-1 proto-oncogene b ELISA Kit, Spi-1 proto-oncogene a ELISA Kit, SPI1 ELISA Kit, Spi1 ELISA Kit, spi1b ELISA Kit, spi1a ELISA Kit
基因ID
6688
途径
Stem Cell Maintenance
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