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PSMA1 ELISA 试剂盒
PSMA1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
PSMA1ELISA Kit详情
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PSMA1
(Proteasome Subunit alpha Type 1 (PSMA1))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Custom Human PSMA1 ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes .
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
PSMA1目标详情
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PSMA1
(Proteasome Subunit alpha Type 1 (PSMA1))
别名
PSMA1 (PSMA1 产品 )
别名
PSMA1 ELISA Kit, pros30 ELISA Kit, DDBDRAFT_0204226 ELISA Kit, DDBDRAFT_0214956 ELISA Kit, DDB_0204226 ELISA Kit, DDB_0214956 ELISA Kit, C2 ELISA Kit, HC2 ELISA Kit, Pros-30 ELISA Kit, alpha-type ELISA Kit, NU ELISA Kit, PROS30 ELISA Kit, CC2 ELISA Kit, zgc:92726 ELISA Kit, 143314_at ELISA Kit, Alpha-6 ELISA Kit, CG4904 ELISA Kit, Dmel\CG4904 ELISA Kit, PROS-35 ELISA Kit, PROS-Dm35 ELISA Kit, Pros-35 ELISA Kit, Pros-Dm35 ELISA Kit, Prosalpha6 ELISA Kit, alpha6 ELISA Kit, alpha_dm ELISA Kit, anon-SAGE:Wang-116 ELISA Kit, pros 35 ELISA Kit, pros35 ELISA Kit, proteasome subunit alpha 1 ELISA Kit, proteasome subunit alpha type 1 ELISA Kit, 20S proteasome subunit C2 ELISA Kit, proteasome (prosome, macropain) subunit, alpha type 1 ELISA Kit, proteasome subunit alpha 1 L homeolog ELISA Kit, Proteasome alpha6 subunit ELISA Kit, PSMA1 ELISA Kit, psma1 ELISA Kit, CNB05540 ELISA Kit, psmA1 ELISA Kit, Psma1 ELISA Kit, psma1.L ELISA Kit, Prosalpha6 ELISA Kit
基因ID
5682
UniProt
P25786
途径
Mitotic G1-G1/S Phases , DNA Replication , Synthesis of DNA
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