KRT8 ELISA 试剂盒
KRT8
适用: 人
Colorimetric
Sandwich ELISA
0.410-100 pg/mL
Cell Culture Supernatant, Plasma, Serum
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- 抗原 See all KRT8 ELISA试剂盒
- KRT8 (Keratin 8 (KRT8))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.410-100 pg/mL
- 最低检测浓度
- 0.410 pg/mL
- 应用范围
- ELISA
- 原理
- Human Cytokeratin 8/KRT8 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Cytokeratin 8.
- 灵敏度
- 0.4 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Keratin 8 (KRT8) ELISA Kit
KRT8 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all KRT8 ELISA试剂盒
- KRT8 (Keratin 8 (KRT8))
- 别名
- Cytokeratin 8 (KRT8 产品)
- 别名
- CARD2 ELISA Kit, CK-8 ELISA Kit, CK8 ELISA Kit, CYK8 ELISA Kit, K2C8 ELISA Kit, K8 ELISA Kit, KO ELISA Kit, AA960620 ELISA Kit, AL022697 ELISA Kit, AU019895 ELISA Kit, Card2 ELISA Kit, EndoA ELISA Kit, Krt-2.8 ELISA Kit, Krt2-8 ELISA Kit, CYKER ELISA Kit, KRT2-8 ELISA Kit, KERATIN8 ELISA Kit, ck8 ELISA Kit, cyk8 ELISA Kit, k2c8 ELISA Kit, card2 ELISA Kit, krt2-5 ELISA Kit, MGC69490 ELISA Kit, KRT8 ELISA Kit, DreK8 ELISA Kit, cb186 ELISA Kit, krt2-8 ELISA Kit, sb:cb186 ELISA Kit, wu:fa20h05 ELISA Kit, wu:fa95h10 ELISA Kit, wu:fb96c06 ELISA Kit, zf-K8 ELISA Kit, zfk8 ELISA Kit, DKFZp468F2127 ELISA Kit, keratin 8 ELISA Kit, keratin 8 S homeolog ELISA Kit, KRT8 ELISA Kit, Krt8 ELISA Kit, krt8.S ELISA Kit, krt8 ELISA Kit
- 基因ID
- 3856
- UniProt
- P05787
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