Pancreatic Prohormone Or Pancreatic Polypeptide (ProPPY, ProPNP) and Pancreatic Icosapeptide (PI) ELISA 试剂盒
适用: 人, 小鼠, 大鼠
Colorimetric
Competition ELISA
0.1-1.000 ng/mL
Cell Culture Supernatant, Plasma, Serum
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- 抗原
- Pancreatic Prohormone Or Pancreatic Polypeptide (ProPPY, ProPNP) and Pancreatic Icosapeptide (PI)
- 适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 0.1-1.000 ng/mL
- 最低检测浓度
- 0.1 ng/mL
- 应用范围
- ELISA
- 原理
- Human/Mouse/Rat ProPPY/PI EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This EIA kit is designed to detect human, mouse, and rat pro-pancreatic polypeptide (Pro-PPY) and pancreatic icosapeptide, not active pancreatic polypeptide.
- 灵敏度
- 0.5 ng/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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-
- 应用备注
- Recommended Dilution for serum and plasma samplesHuman: 2X / Mouse: 2X / Rat: 2X
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- Standard, Biotinylated ProPPY peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
- 有效期
- 6 months
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- 抗原
- Pancreatic Prohormone Or Pancreatic Polypeptide (ProPPY, ProPNP) and Pancreatic Icosapeptide (PI)
- 基因ID
- 5539
- UniProt
- P01298
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