SERPINA1 ELISA 试剂盒
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- 抗原 See all SERPINA1 ELISA试剂盒
- SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 3.12-200 ng/mL
- 最低检测浓度
- 3.12 ng/mL
- 应用范围
- ELISA
- 原理
- This immunoassay kit allows for the in vitro quantitative determination of human ? 1-Antitrypsin, ? 1-AT concentrations in cell culture supernates, serum, plasma and other biological fluids.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay recognizes recombinant and natural human ? 1-AT.
- 交叉反应 （详细）
- No significant cross-reactivity or interference was observed.
- 灵敏度
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< 9.75 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. - 产品特性
- Homo sapiens,Human,Alpha-1-antitrypsin,Alpha-1 protease inhibitor,Alpha-1-antiproteinase,Serpin A1,SERPINA1,AAT,PI,PRO0684,PRO2209
- Featured
- Discover our best selling SERPINA1 ELISA Kit
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- Discover our top product SERPINA1 ELISA Kit
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serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA Kit
SERPINA1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 人 Colorimetric Sandwich ELISA 7.81 ng/mL - 500 ng/mL Plasma, Serum, Urine
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 200 ng/mL Plasma, Serum, Urine
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSmall Sample SERPINA1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 人 Colorimetric Sandwich ELISA 0.391-100 ng/mL Cell Culture Cells, Cerebrospinal Fluid, Milk, Saliva, Urine
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to α 1-AT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for α 1-AT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain α 1-AT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a 2 sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of α 1-AT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
-
- 抗原 See all SERPINA1 ELISA试剂盒
- SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
- 别名
- SERPINA1 (SERPINA1 产品)
- 别名
- AACT ELISA Kit, ACT ELISA Kit, GIG25 ELISA Kit, A1AT ELISA Kit, PI ELISA Kit, alpha-1-PI ELISA Kit, A1A ELISA Kit, AAT ELISA Kit, PI1 ELISA Kit, PRO2275 ELISA Kit, alpha1AT ELISA Kit, Pi ELISA Kit, Spi1 ELISA Kit, MGC64429 ELISA Kit, serpina1d ELISA Kit, Aps ELISA Kit, SERPINA3-5 ELISA Kit, serpin family A member 3 ELISA Kit, alpha-1-antiproteinase F precursor ELISA Kit, serpin family A member 1 ELISA Kit, alpha-1-antitrypsin ELISA Kit, calumenin ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 S homeolog ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 ELISA Kit, SH2B adaptor protein 2 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 L homeolog ELISA Kit, SERPINA3 ELISA Kit, LOC100008973 ELISA Kit, SERPINA1 ELISA Kit, LOC100328621 ELISA Kit, CALU ELISA Kit, Serpina1 ELISA Kit, serpina1.S ELISA Kit, serpina1 ELISA Kit, SPIA5 ELISA Kit, Sh2b2 ELISA Kit, Calu ELISA Kit, serpina1.L ELISA Kit
- 背景
- Alpha 1-Antitrypsin or α 1-antitrypsin (A1AT) is a glycoprotein and generally known as serum trypsin inhibitor. The correct name, however, is alpha-1 proteinase inhibitor (A1PI) because it is a serine protease inhibitor (serpin) inhibiting a wide variety of proteases. It protects tissues from enzymes of inflammatory cells, especially elastase, and is present in human blood at 1.5 - 3.5 gram/liter, but the concentration can rise manyfolds upon acute inflammation. In its absence, elastase is free to break down elastin -- which contributes to the elasticity of the lungs -- resulting in respiratory complications such as emphysema leading finally to COPD (chronic obstructive pulmonary disease). A1AT is a 52 kDa serpin (serine protease inhibitor), and in medicine it is considered the most prominent serpin, given the fact that the words α 1-antitrypsin and protease inhibitor (Pi) are often used interchangeably. Most serpins inactivate enzymes by binding to them covalently, requiring very high levels to perform their function. In the acute phase reaction, a further elevation is required to "limit" the damage caused by activated neutrophil granulocytes and their enzyme elastase, which breaks down the connective tissue fiber elastin. Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins which can polymerise and accumulate in the liver (infantile hepatic cirrhosis).
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