Anti-PAR2 Antibody (IgG) ELISA 试剂盒
Colorimetric
Competition ELISA
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- 抗原
- Anti-PAR2 Antibody (IgG)
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- 原理
- The anti-alpha2-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the Alpha2-adrenergic-receptor in serum and plasma.
- Analytical Method
- Quantitative
- 产品特性
- The anti-Thrombin-Receptor-like-1-Antibody-EIA is an antibody screening test.
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- 板类型
- Pre-coated
- 实验流程
- The Thrombin-Receptor-like-1 has been pre-coated onto a microtiter plate. During the first incubation the anti-Thrombin-Receptor-like-1-Antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-Thrombin-Receptor-like-1-Antibody.
- 实验流程
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Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add Stopp solution: 100 µL/well
Read at 450nm - 实验精密度
- Intraassay-Precision:7,83% Interassay-Precision:9,12%
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原
- Anti-PAR2 Antibody (IgG)
- 物质类
- Antibody
- 背景
- The Alpha-2 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP.
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