FAK ELISA 试剂盒
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- 抗原 See all FAK (PTK2) ELISA试剂盒
- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
- 抗原表位
- pTyr397, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-FAK (Y397) and Total ELISA Kit. This assay semi-quantitatively measures phophorylated FAK (Tyr397) and Total FAK in lysate samples.
- 样品类型
- Cell Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human FAK phosphorylated at site Tyrosine-397 and Total FAK.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product PTK2 ELISA Kit
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all FAK (PTK2) ELISA试剂盒
- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
- 别名
- FAK (PTK2 产品)
- 别名
- FADK ELISA Kit, FAK ELISA Kit, FAK1 ELISA Kit, FRNK ELISA Kit, PPP1R71 ELISA Kit, p125FAK ELISA Kit, pp125FAK ELISA Kit, PTK2 ELISA Kit, Fadk ELISA Kit, mKIAA4203 ELISA Kit, fak ELISA Kit, fak1a ELISA Kit, ptk2 ELISA Kit, ptk2a ELISA Kit, wu:fa18c04 ELISA Kit, zgc:66114 ELISA Kit, CG10023 ELISA Kit, CT28129 ELISA Kit, DFAK ELISA Kit, DFak ELISA Kit, DFak56 ELISA Kit, Dfak ELISA Kit, Dfak56 ELISA Kit, DmFAK ELISA Kit, Dmel\\CG10023 ELISA Kit, Fak56 ELISA Kit, Fak56D ELISA Kit, fak56 ELISA Kit, protein tyrosine kinase 2 ELISA Kit, protein tyrosine kinase 2 L homeolog ELISA Kit, PTK2 protein tyrosine kinase 2 ELISA Kit, protein tyrosine kinase 2ab ELISA Kit, Focal adhesion kinase ELISA Kit, PTK2 ELISA Kit, ptk2.L ELISA Kit, Ptk2 ELISA Kit, ptk2ab ELISA Kit, Fak ELISA Kit
- 背景
- FAK phosohorylated at Tyrosine-397 and Total FAK
- 基因ID
- 5747
- UniProt
- Q05397
- 途径
- Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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