RAS CLIA Kit
适用: 人
Chemiluminescent
Sandwich ELISA
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- 抗原 See all RAS products
- RAS
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Ras GTPase Chemi ELISA Kit is the first ELISA-based kit designed to detect and quantify activated Ras GTPase.
- Analytical Method
- Quantitative
- 特异性
- Ras GTPase Chemi ELISAs are tested for sensitivity in detecting activated Ras.
- 组件
- 96-well glutathione-coated assay plate with plate sealer, GST-Raf-RBD, primary antibody, HRP-conjugated secondary antibody, positive control cell extract, Protease Inhibitor Cocktail, Lysis and Reaction buffers.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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25 μg of whole-cell extracts from unstimulated 293T/17 cells and HeLa cells stimulated with 5 ng/ml EGF for 2 minutes per well were assayed using the Ras GTPase Chemi ELISA Kit (Figure 1). For lot 05715007, the signals were read on a luminometer within 15 minutes of adding the chemiluminescent reaction buffer. For the luminometer, the gain was set at 150 and the integration time was set to maximum. The level of Ras activation, and the ratio between the stimulated and unstimulated samples may vary depending on the cell type tested and the treatment used.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
- Store the extract and the GST-Raf-RBD protein at -80°C, the antibodies and protease inhibitors should be stored at -20°C. All other kit components must be stored at 4°C prior to use. This product is guaranteed for 6 months from date of receipt.
- 有效期
- 6 months
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- 抗原 See all RAS products
- RAS
- Abstract
- RAS 产品
- 别名
- asr CLIA Kit, resistance to audiogenic seizures CLIA Kit, ras CLIA Kit
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