ADAMTS1 ELISA 试剂盒
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- 抗原 See all ADAMTS1 ELISA试剂盒
- ADAMTS1 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))
- 抗原表位
- AA 253-734
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 93.8-6000 pg/mL
- 最低检测浓度
- 93.8 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAMTS1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: F253-A734 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: F253-A734 - Featured
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ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1) ELISA Kit
ADAMTS1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1) ELISA KitADAMTS1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1) ELISA KitADAMTS1 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1) ELISA KitADAMTS1 适用: 大鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1) ELISA KitADAMTS1 适用: 人, 小鼠 Colorimetric Sandwich ELISA 0.4-100 ng/mL Cell Culture Supernatant, Plasma, Serum
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1) ELISA KitADAMTS1 适用: 人 Colorimetric Sandwich ELISA 0.312-20 ng/mL Plasma, Serum, Tissue Homogenate, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 1 disintegrin domain.
- 板类型
- Pre-coated
- 实验流程
- human ADAMTS1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS1 has been precoated onto 96-well plates. Standards(NSO, F253-A734) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human ADAMTS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ADAMTS1 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 1208, Standard deviation: 42.28, CV(%): 3.5
- Sample 2: n=16, Mean(pg/ml): 2741, Standard deviation: 117.9, CV(%): 4.3
- Sample 3: n=16, Mean(pg/ml): 4137, Standard deviation: 202.7, CV(%): 4.9,
- Sample 1: n=24, Mean(pg/ml): 1850, Standard deviation: 98.05, CV(%): 5.3
- Sample 2: n=24, Mean(pg/ml): 3035, Standard deviation: 176.03, CV(%): 5.8
- Sample 3: n=24, Mean(pg/ml): 4726, Standard deviation: 302.5, CV(%): 6.4
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "siRNA-knockdown of ADAMTS-13 modulates endothelial cell angiogenesis." in: Microvascular research, Vol. 113, pp. 65-70, (2018) (PubMed).
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: "siRNA-knockdown of ADAMTS-13 modulates endothelial cell angiogenesis." in: Microvascular research, Vol. 113, pp. 65-70, (2018) (PubMed).
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- 抗原 See all ADAMTS1 ELISA试剂盒
- ADAMTS1 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))
- 别名
- ADAMTS1 (ADAMTS1 产品)
- 别名
- c3-c5 ELISA Kit, meth1 ELISA Kit, xadamts1 ELISA Kit, C3-C5 ELISA Kit, METH1 ELISA Kit, ADAM-TS1 ELISA Kit, ADAMTS ELISA Kit, ADAMTS-1 ELISA Kit, METH-1 ELISA Kit, adamts1 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif 1 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif, 1 ELISA Kit, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif 1 L homeolog ELISA Kit, ADAMTS1 ELISA Kit, adamts1 ELISA Kit, Adamts1 ELISA Kit, adamts1.L ELISA Kit
- 背景
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Protein Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. .
Background: ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What’s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.
Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 1,ADAM-TS 1,ADAM-TS1,ADAMTS-1,3.4.24.-,METH-1,ADAMTS1,KIAA1346, METH1,
Full Gene Name: A disintegrin and metalloproteinase with thrombospondin motifs 1
Cellular Localisation: Secreted, extracellular space, extracellular matrix. - 基因ID
- 9510
- UniProt
- Q9UHI8
- 途径
- SARS-CoV-2 Protein Interactome
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