ADAMTS13 ELISA 试剂盒
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- 抗原 See all ADAMTS13 ELISA试剂盒
- ADAMTS13 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))
- 抗原表位
- AA 34-688
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78-50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAMTS13
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
- CHO, Q34-W688
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <20pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: CHO
Immunogen sequence: Q34-W688 - Featured
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ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA Kit
ADAMTS13 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 人 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Plasma. Expressed primarily in liver. .
- 板类型
- Pre-coated
- 实验流程
- human ADAMTS13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS13 has been precoated onto 96-well plates. Standards(CHO, Q34-W688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS13 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human ADAMTS13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human ADAMTS13 standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 5.62, Standard deviation: 0.259, CV(%): 4.6
- Sample 2: n=16, Mean(ng/ml): 10.1, Standard deviation: 0.444, CV(%): 4.4
- Sample 3: n=16, Mean(ng/ml): 25.5, Standard deviation: 1.07, CV(%): 4.2,
- Sample 1: n=24, Mean(ng/ml): 6.23, Standard deviation: 0.467, CV(%): 7.5
- Sample 2: n=24, Mean(ng/ml): 12.1, Standard deviation: 0.835, CV(%): 6.9
- Sample 3: n=24, Mean(ng/ml): 26.4, Standard deviation: 1.95, CV(%): 7.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all ADAMTS13 ELISA试剂盒
- ADAMTS13 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))
- 别名
- ADAMTS13 (ADAMTS13 产品)
- 别名
- ADAMTS13 ELISA Kit, ADAM-TS13 ELISA Kit, ADAMTS-13 ELISA Kit, C9orf8 ELISA Kit, VWFCP ELISA Kit, vWF-CP ELISA Kit, Gm710 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif 13 ELISA Kit, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif, 13 ELISA Kit, ADAMTS13 ELISA Kit, Adamts13 ELISA Kit, adamts13 ELISA Kit
- 背景
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Protein Function: Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. .
Background: ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.
Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 13,ADAM-TS 13,ADAM-TS13,ADAMTS-13,3.4.24.87,von Willebrand factor-cleaving protease,vWF-CP,vWF-cleaving protease,ADAMTS13,C9orf8,UNQ6102/PRO20085,
Full Gene Name: A disintegrin and metalloproteinase with thrombospondin motifs 13
Cellular Localisation: Secreted . Secretion enhanced by O-fucosylation of TSP type-1 repeats. - 基因ID
- 11093
- UniProt
- Q76LX8
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