NBL1 ELISA 试剂盒
-
- 抗原 See all NBL1 ELISA试剂盒
- NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))
- 抗原表位
- AA 17-181
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 125-8000 pg/mL
- 最低检测浓度
- 125 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DAN/NBL1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- NSO, A17-D181
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: A17-D181 - Featured
- Discover our best selling NBL1 ELISA Kit
- Top Product
- Discover our top product NBL1 ELISA Kit
-
Neuroblastoma 1, DAN Family BMP Antagonist (NBL1) ELISA Kit
NBL1 适用: 人 Colorimetric Sandwich ELISA 34.4 pg/mL - 2200 pg/mL Plasma, Serum, Tissue Homogenate
Neuroblastoma 1, DAN Family BMP Antagonist (NBL1) ELISA KitNBL1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Neuroblastoma 1, DAN Family BMP Antagonist (NBL1) ELISA KitNBL1 适用: 小鼠 AA 17-178 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Neuroblastoma 1, DAN Family BMP Antagonist (NBL1) ELISA KitNBL1 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Neuroblastoma 1, DAN Family BMP Antagonist (NBL1) ELISA KitNBL1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
-
- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Tissue Specificity: Most abundant in normal lung and meningioma.
- 板类型
- Pre-coated
- 实验流程
- human DAN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DAN has been precoated onto 96-well plates. Standards (NSO, A17-D181) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DAN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DAN amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human DAN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human DAN standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 1257, Standard deviation: 72.90, CV(%): 5.8
- Sample 2: n=16, Mean(pg/ml): 3204, Standard deviation: 189.03, CV(%): 5.9
- Sample 3: n=16, Mean(pg/ml): 5394, Standard deviation: 334.42, CV(%): 6.2,
- Sample 1: n=24, Mean(pg/ml): 1020, Standard deviation: 65.28, CV(%): 6.4
- Sample 2: n=24, Mean(pg/ml): 3562, Standard deviation: 235.09, CV(%): 6.6
- Sample 3: n=24, Mean(pg/ml): 4975, Standard deviation: 353.22, CV(%): 7.1
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all NBL1 ELISA试剂盒
- NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))
- 别名
- NBL1 (NBL1 产品)
- 别名
- D1S1733E ELISA Kit, DAN ELISA Kit, DAND1 ELISA Kit, NB ELISA Kit, NO3 ELISA Kit, D4H1S1733E ELISA Kit, Dana ELISA Kit, dand1 ELISA Kit, nbl1-a ELISA Kit, no3 ELISA Kit, 4.1N ELISA Kit, Epb41l1 ELISA Kit, NBL1 ELISA Kit, mKIAA0338 ELISA Kit, C1orf151-NBL1 ELISA Kit, dan ELISA Kit, zgc:77251 ELISA Kit, neuroblastoma 1, DAN family BMP antagonist ELISA Kit, neuroblastoma, suppression of tumorigenicity 1 ELISA Kit, neuroblastoma 1, DAN family BMP antagonist L homeolog ELISA Kit, neuroblastoma suppressor of tumorigenicity 1 ELISA Kit, erythrocyte membrane protein band 4.1 like 1 ELISA Kit, MINOS1-NBL1 readthrough ELISA Kit, NBL1 ELISA Kit, Nbl1 ELISA Kit, nbl1.L ELISA Kit, LOC535280 ELISA Kit, Epb41l1 ELISA Kit, MINOS1-NBL1 ELISA Kit, nbl1 ELISA Kit
- 背景
-
Protein Function: Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.
Synonyms: Neuroblastoma suppressor of tumorigenicity 1,DAN domain family member 1,Protein N03,Zinc finger protein DAN,NBL1,DAN, DAND1,
Full Gene Name: Neuroblastoma suppressor of tumorigenicity 1
Cellular Localisation: Secreted. - 基因ID
- 4681
- UniProt
- P41271
-
