TGFBR3 ELISA 试剂盒
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- TGFBR3 (Transforming Growth Factor, beta Receptor III (TGFBR3))
- 抗原表位
- AA 21-781
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10.000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TGFBR3
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- NSO, G21-D781
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: G21-D781 - Featured
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Transforming Growth Factor, beta Receptor III (TGFBR3) ELISA Kit
TGFBR3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta Receptor III (TGFBR3) ELISA KitTGFBR3 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human TGFBR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGFBR3 has been precoated onto 96-well plates. Standards(NSO,G21-D781) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFBR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGFBR3 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TGFBR3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TGFBR3 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.15, Standard deviation: 0.051, CV(%): 4.5
- Sample 2: n=16, Mean(ng/ml): 2.52, Standard deviation: 0.108, CV(%): 4.3
- Sample 3: n=16, Mean(ng/ml): 5.81, Standard deviation: 0.284, CV(%): 4.9,
- Sample 1: n=24, Mean(ng/ml): 1.63, Standard deviation: 0.109, CV(%): 6.7
- Sample 2: n=24, Mean(ng/ml): 2.87, Standard deviation: 0.169, CV(%): 5.9
- Sample 3: n=24, Mean(ng/ml): 5.66, Standard deviation: 0.401, CV(%): 7.1
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- TGFBR3 (Transforming Growth Factor, beta Receptor III (TGFBR3))
- 别名
- TGFBR3 (TGFBR3 产品)
- 别名
- TGFBR3 ELISA Kit, DKFZp469I0915 ELISA Kit, BGCAN ELISA Kit, betaglycan ELISA Kit, 1110036H20Rik ELISA Kit, AU015626 ELISA Kit, AW215636 ELISA Kit, TBRIII ELISA Kit, transforming growth factor beta receptor 3 ELISA Kit, transforming growth factor, beta receptor III ELISA Kit, TGFBR3 ELISA Kit, tgfbr3 ELISA Kit, Tgfbr3 ELISA Kit
- 背景
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Protein Function: Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors.
Background: Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell- surfacechondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. Betaglycan binds to various members of the TGF-beta superfamily of ligands via its core protein, and bFGF via its heparan sulfate chains. It is not involved directly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. By cDNA array and immunohistochemistry analyses, it is found that TGFBR3 expression is lost in most breast cancers examined in association with loss of heterozygosity of the TGFBR3 locus. TGFBR3 expression decreased during breast cancer progression, and low levels predicted decreased recurrence-free survival in patients. Loss of TGFBR3 through allelic imbalance is a frequent genetic event during breast cancer development that increases metastatic potential. Also, TGFBR3 can localize transformation in the heart and play an essential, nonredundant role in TGF-beta signaling.
Synonyms: Transforming growth factor beta receptor type 3,TGF-beta receptor type 3,TGFR-3,Betaglycan,Transforming growth factor beta receptor III,TGF-beta receptor type III,TGFBR3,
Full Gene Name: Transforming growth factor beta receptor type 3
Cellular Localisation: Secreted. Secreted, extracellular space. Cell membrane, Single-pass type I membrane protein . Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix. - 基因ID
- 7049
- UniProt
- Q03167
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