IGF1 ELISA 试剂盒
(4 references)Quick Overview for IGF1 ELISA 试剂盒 (ABIN2859206)
抗原
See all IGF1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- For quantitative detection of human IGF-1 in cell culture supernates, serum and plasma(heparin, EDTA).
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant human IGF-1
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交叉反应 （详细）
- There is no detectable cross-reactivity with IGF-2.
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灵敏度
- <10pg/mL
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组件
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- 96-well plate precoated with anti- human IGF-1 antibod - 1
- Lyophilized recombinant human IGF-1 standar - 10ng/tubex2
- Biotinylated anti- human IGF-1 antibod - 130μl(dilution 1:100)
- Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
- Sample diluent buffe - 30 ml
- Antibody diluent buffe - 12ml
- ABC diluent buffe - 12ml
- TMB color developing agen - 10ml
- TMB stop solutio - 10ml
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: G49-A118
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Insulin-Like Growth Factor 1 (IGF1) ELISA Kit
IGF1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 犬 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 大鼠 AA 49-118 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: Cow Colorimetric Competition ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitIGF1 适用: 鸭 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 (IGF1) ELISA KitHigh Sensitivity IGF1 适用: 人 Colorimetric Sandwich ELISA 0.19 ng/mL - 12 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- human IGF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-1 has been precoated onto 96-well plates. Standards(E.coli, G49-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGF-1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGF-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGF-1 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 523, Standard deviation: 21.97, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 1345, Standard deviation: 65.91, CV(%): 4.9
- Sample 3: n=16, Mean(pg/ml): 2632, Standard deviation: 134.2, CV(%): 5.1,
- Sample 1: n=24, Mean(pg/ml): 645, Standard deviation: 37.41, CV(%): 5.8
- Sample 2: n=24, Mean(pg/ml): 1631, Standard deviation: 109.3, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 3045, Standard deviation: 231.4, CV(%): 7.6
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限制
- 仅限研究用
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." in: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).
: "Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via activating JNK signal." in: PLoS ONE, Vol. 8, Issue 5, pp. e63561, (2013) (PubMed).
: "IGF-1 promotes the development and cytotoxic activity of human NK cells." in: Nature communications, Vol. 4, pp. 1479, (2013) (PubMed).
: "Antibacterial effect of 317L stainless steel contained copper in prevention of implant-related infection in vitro and in vivo." in: Journal of materials science. Materials in medicine, Vol. 22, Issue 11, pp. 2525-35, (2011) (PubMed).
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: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." in: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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别名
- IGF1
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背景
- Insulin-like growth factor 1(IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD.1 The IGF1 gene, mapped on 12q22-q24. contains 5 exons. Exons 1-4 encode the 195-amino acid precursor(IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide(IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98 % of IGF-1 is always bound to one of 6 binding proteins(IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
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基因ID
- 3479
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UniProt
- P05019
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途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
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