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IgG2a ELISA 试剂盒

适用: 小鼠 Colorimetric Sandwich ELISA 0.6-150 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2703581
发货至: 中国
  • 抗原 See all IgG2a products
    IgG2a
    适用
    • 3
    • 3
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.6-150 ng/mL
    最低检测浓度
    0.6 ng/mL
    应用范围
    ELISA
    原理
    Mouse IgG2a ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IgG2a in serum, plasma, and cell culture supernatants.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair detects mouse heavy chain of IgG2a. Other species not determined.
    灵敏度
    0.6 ng/ml
    组件
    96 wells (12 strips x 8 wells) Coated With Antibody
    Wash Buffer Concentrate (20x)
    Positive Control
    Assay Diluent
    Detection Antibody
    Secondary Antibody or HRP Streptavidin
    TMB One-Step Substrate Reagent
    Stop Solution
    Cell Lysis Buffer
    试剂未包括
    Distilled or deionized water
    100 mL and 1 liter graduated cylinders
    Tubes to prepare sample dilutions
    Protease and Phosphatase inhibitors
    Precision pipettes to deliver 2 μL to 1 mL volumes
    Adjustable 1-25 mL pipettes for reagent preparation
    Benchtop rocker or shaker
    Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Discover our best selling IgG2a ELISA Kit
  • 应用备注
    Optimal working dilution: 10,000 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    This assay employs an antibody specific for mouse IgG2a coated on a 96-well plate. Standards and samples are pipetted into the wells and IgG2a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IgG2a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG2a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
    样品制备

    For example, add 2 μL of serum or plasma into a tube with 198.0 μL 1X Assay Diluent to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 3 μL of prepared 100-fold diluted sample into a tube with 297 μL 1X Assay Dil

    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all IgG2a products
    IgG2a
    Abstract
    IgG2a 产品
    别名
    gamma-2a immunoglobulin heavy chain ELISA Kit, IgG-2a ELISA Kit
    物质类
    Antibody
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