IgA ELISA 试剂盒
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- 抗原 See all IgA ELISA试剂盒
- IgA
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.5-500 ng/mL
- 最低检测浓度
- 1.5 ng/mL
- 应用范围
- ELISA
- 原理
- Mouse IgA ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects mouse IgA. Other species not determined.
- 灵敏度
- 1.5 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product IgA ELISA Kit
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IgA ELISA Kit
适用: Cow Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgA ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
IgA ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.14 ng/mL - 100 ng/mL Plasma, Saliva, Serum
IgA ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
IgA ELISA Kit适用: 小鸡 Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Plasma, Serum
IgA ELISA Kit适用: 绵羊 Colorimetric Competition ELISA 3.75 μg/mL - 60 μg/mL Plasma, Serum
IgA ELISA Kit适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
IgA ELISA Kit适用: 小鸡 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
IgA ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgA ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 1000 ng/mL Plasma, Serum, Urine
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- 应用备注
- Recommended Dilution for serum and plasma samples20,000 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
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For example, add 2 μL of serum or plasma into a tube with 198.0 μL 1X Assay Diluent to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 398 μL 1X Assay Dil
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all IgA ELISA试剂盒
- IgA
- Abstract
- IgA 产品
- 别名
- MB-1 ELISA Kit, IG-alpha ELISA Kit, IGA ELISA Kit, IgA ELISA Kit, Igh-2 ELISA Kit, CD79a molecule ELISA Kit, immunoglobulin heavy constant alpha ELISA Kit, CD79A ELISA Kit, Igha ELISA Kit
- 物质类
- Antibody
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