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HAVCR1 ELISA 试剂盒
HAVCR1
适用: 大鼠
Colorimetric
Sandwich ELISA
10-3.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
HAVCR1ELISA Kit详情
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HAVCR1
(Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
适用
All reactivities for HAVCR1 ELISA试剂盒
大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
10-3.000 pg/mL
最低检测浓度
10 pg/mL
应用范围
ELISA
原理
Rat TIM-1 (KIM-1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
样品类型
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
特异性
This ELISA antibody pair detects rat TIM-1, no cross-reactivity with mouse TIM 1, TIM-3, TIM-4, human TIM 1 or TIM-4 is observed.
灵敏度
10 pg/mL
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
HAVCR1目标详情
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HAVCR1
(Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
别名
TIM-1 (HAVCR1 产品 )
别名
HAVCR ELISA Kit, HAVCR-1 ELISA Kit, KIM-1 ELISA Kit, KIM1 ELISA Kit, TIM ELISA Kit, TIM-1 ELISA Kit, TIM1 ELISA Kit, TIMD-1 ELISA Kit, TIMD1 ELISA Kit, Kim1 ELISA Kit, HAVCR1 ELISA Kit, LOC100226241 ELISA Kit, AI503787 ELISA Kit, Tim1 ELISA Kit, Timd1 ELISA Kit, hepatitis A virus cellular receptor 1 ELISA Kit, hepatitis A virus cellular receptor 1 homolog ELISA Kit, HAVCR1 ELISA Kit, Havcr1 ELISA Kit, LOC100226241 ELISA Kit
物质类
Virus
背景
Gene Names: Havcr1 Kim1 Protein names: Hepatitis A virus cellular receptor 1 homolog (HAVcr-1) (Kidney injury molecule 1) (KIM-1) (T cell immunoglobulin and mucin domain-containing protein 1) (TIMD-1)
基因ID
286934
UniProt
O54947
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