SERPINA1 ELISA 试剂盒
-
- 抗原 See all SERPINA1 ELISA试剂盒
- SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 2-500 ng/mL
- 最低检测浓度
- 2 ng/mL
- 应用范围
- ELISA
- 原理
- Human SERPINA1 (Alpha-1-Antitrypsin) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human Serpin A1. Other species not determined.
- 灵敏度
- 2 ng/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling SERPINA1 ELISA Kit
- Top Product
- Discover our top product SERPINA1 ELISA Kit
-
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA Kit
SERPINA1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 人 Colorimetric Sandwich ELISA 7.81 ng/mL - 500 ng/mL Plasma, Serum, Urine
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 200 ng/mL Plasma, Serum, Urine
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSmall Sample SERPINA1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) ELISA KitSERPINA1 适用: 人 Colorimetric Sandwich ELISA 0.391-100 ng/mL Cell Culture Cells, Cerebrospinal Fluid, Milk, Saliva, Urine
-
- 应用备注
- Recommended Dilution for serum and plasma samples3-30 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all SERPINA1 ELISA试剂盒
- SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
- 别名
- Serpin A1 (SERPINA1 产品)
- 别名
- AACT ELISA Kit, ACT ELISA Kit, GIG25 ELISA Kit, A1AT ELISA Kit, PI ELISA Kit, alpha-1-PI ELISA Kit, A1A ELISA Kit, AAT ELISA Kit, PI1 ELISA Kit, PRO2275 ELISA Kit, alpha1AT ELISA Kit, Pi ELISA Kit, Spi1 ELISA Kit, MGC64429 ELISA Kit, serpina1d ELISA Kit, Aps ELISA Kit, SERPINA3-5 ELISA Kit, serpin family A member 3 ELISA Kit, alpha-1-antiproteinase F precursor ELISA Kit, serpin family A member 1 ELISA Kit, alpha-1-antitrypsin ELISA Kit, calumenin ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 S homeolog ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 ELISA Kit, SH2B adaptor protein 2 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 L homeolog ELISA Kit, SERPINA3 ELISA Kit, LOC100008973 ELISA Kit, SERPINA1 ELISA Kit, LOC100328621 ELISA Kit, CALU ELISA Kit, Serpina1 ELISA Kit, serpina1.S ELISA Kit, serpina1 ELISA Kit, SPIA5 ELISA Kit, Sh2b2 ELISA Kit, Calu ELISA Kit, serpina1.L ELISA Kit
- 背景
-
Gene Names: SERPINA1 AAT PI PRO0684 PRO2209
Protein names: Alpha-1-antitrypsin (Alpha-1 protease inhibitor) (Alpha-1-antiproteinase) (Serpin A1) [Cleaved into: Short peptide from AAT (SPAAT)]
- 基因ID
- 5265
- UniProt
- P01009
-
