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IFNAR2 ELISA 试剂盒

IFNAR2 适用: 人 Colorimetric Sandwich ELISA 0.16-40 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2703098
发货至: 中国
  • 抗原 See all IFNAR2 ELISA试剂盒
    IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))
    适用
    • 4
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.16-40 ng/mL
    最低检测浓度
    0.16 ng/mL
    应用范围
    ELISA
    原理
    Human IFN-alpha / beta R2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM9, CD244, APRIL, BMP-2, BMP-9, Cathepsin L, CD200, CD97, Chemerin, C5a, DcR3, FABP2, FAP, FGF-19, Galectin-3, HGFR, FABP2, FAP, FGF-19, IFG-2R, IL-1R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV, Osteoactivin, PG-1, PGRPs, SerpinA4, sFRP-3, Thrombomodulin, TLR2, Transferrin, WIF-1
    灵敏度
    0.16 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all IFNAR2 ELISA试剂盒
    IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))
    别名
    IFN alpha/beta R2 (IFNAR2 产品)
    别名
    AI747302 ELISA Kit, Ifnar-2 ELISA Kit, IFN-R ELISA Kit, IFN-alpha-REC ELISA Kit, IFNABR ELISA Kit, IFNARB ELISA Kit, IFN-R-2 ELISA Kit, IFNBR ELISA Kit, interferon alpha and beta receptor subunit 2 ELISA Kit, interferon (alpha and beta) receptor 2 ELISA Kit, IFNAR2 ELISA Kit, Ifnar2 ELISA Kit
    背景
    Gene Names: IFNAR2 IFNABR IFNARB
    Protein names: Interferon alpha/beta receptor 2 (IFN-R-2) (IFN-alpha binding protein) (IFN-alpha/beta receptor 2) (Interferon alpha binding protein) (Type I interferon receptor 2)
    基因ID
    3455
    UniProt
    P48551
    途径
    JAK/STAT Signaling, Hepatitis C
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