SPINT2 ELISA 试剂盒
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- 抗原 See all SPINT2 ELISA试剂盒
- SPINT2 (serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 3-800 pg/mL
- 最低检测浓度
- 3 pg/mL
- 应用范围
- ELISA
- 原理
- Human HAI-2 ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended as an anticoagulant for use in this assay.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human HAI-2, less than 0.1 % cross-reactivity with human HAI-1 and mouse HAI-2B is observed
- 灵敏度
- 3 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2) ELISA Kit
SPINT2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2) ELISA KitSPINT2 适用: 小鼠 AA 30-140 Colorimetric Sandwich ELISA
serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2) ELISA KitSPINT2 适用: 人 AA 1-197 Colorimetric Sandwich ELISA
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
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Collect plasma using EDTA and/or Citrate as anticoagulants. Heparin is not recommended as an anticoagulant for use in this assay.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all SPINT2 ELISA试剂盒
- SPINT2 (serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2))
- 别名
- HAI-2 (SPINT2 产品)
- 别名
- HAI-2 ELISA Kit, Pb ELISA Kit, DIAR3 ELISA Kit, HAI2 ELISA Kit, Kop ELISA Kit, PB ELISA Kit, AL024025 ELISA Kit, C76321 ELISA Kit, serine peptidase inhibitor, Kunitz type, 2 ELISA Kit, serine peptidase inhibitor, Kunitz type 2 ELISA Kit, serine protease inhibitor, Kunitz type 2 ELISA Kit, Spint2 ELISA Kit, SPINT2 ELISA Kit
- 背景
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Gene Names: SPINT2 HAI2 KOP
Protein names: Kunitz-type protease inhibitor 2 (Hepatocyte growth factor activator inhibitor type 2) (HAI-2) (Placental bikunin)
- 基因ID
- 10653
- UniProt
- O43291
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