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FAS ELISA 试剂盒

This 大鼠 FAS ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 FAS.
产品编号 ABIN2702995
发货至: 中国

Quick Overview for FAS ELISA 试剂盒 (ABIN2702995)

抗原

See all FAS ELISA试剂盒
FAS (TNF Receptor Superfamily, Member 6 (FAS))

适用

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大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

40-10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum
  • 最低检测浓度

    40 pg/mL

    原理

    Rat Fas (TNFRSF6/Apo-1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

    Analytical Method

    Quantitative

    特异性

    The antibody pair provided in this kit recognizes Rat FAS.

    灵敏度

    40 pg/mL

    产品特性

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    试剂未包括

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注

    Recommended Dilution for serum and plasma samples2 fold

    样本量

    100 μL

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    -20 °C

    储存方法

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    有效期

    6 months
  • 抗原 See all FAS ELISA试剂盒

    FAS (TNF Receptor Superfamily, Member 6 (FAS))

    别名

    Fas

    背景

    Gene Names: Fas Apt1 Tnfrsf6
    Protein names: Tumor necrosis factor receptor superfamily member 6 (Apo-1 antigen) (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD antigen CD95)

    基因ID

    246097

    UniProt

    Q63199

    途径

    p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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